Humid vs. dry embryo culture conditions on embryo development: a continuous embryo monitoring assessment

Embryo Culture Challenge: Microbial Contamination

The success rate of embryo production critically depends on several factors. One of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (IVF) and in vitro embryo culture (IVC) (1, 2). Besides the technique selected for IVF / in vitro embryo production (IVEP), there is an important consideration called IVF/IVEP culture dishes disin...

Embryo culture, assessment, selection and transfer

Human chorionic gonadotropin from day 2 spent embryo culture media and its relationship to embryo development.

OBJECTIVE To detect hCG in spent embryo culture media at day 2 after intracytoplasmic sperm injection and to assess the relationship of hCG to embryo development. DESIGN Experimental study. SETTING Fertility center and clinical diagnostic laboratory. SAMPLE(S) A total of 102 spent culture media from day 2 human embryos and corresponding unexposed media for blank control. INTERVENTION(S)...

In Vivo Embryo Culture Device

Z.P. Nagy et al. (eds.), Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, DOI 10.1007/978-1-4419-1780-5_19, © Springer Science+Business Media, LLC 2012 Abstract The INVO procedure using the INVOcell device is a simple technique of human assisted reproduction. The fertilization of the oocytes and the early development of the embryos occur in the INVOcell device pla...

Effect of Two Polarized Culture System Prepared From Human Female Genital Tract on Mouse Embryo Development

Purpose: To compare effects of polarized epithelial monolayer prepared from human oviduct and uterus on mouse one and two-cell embryo development. Materials and Methods: Human oviduct and endometrial tissue was obtained from patients who had undergone total hysterectomy. The epithelial cells were isolated from tissue and cultured on extracellular matrix (ECM) Gel coated Millipore filter insert...